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. 2014 Feb 3;9(2):e87888. doi: 10.1371/journal.pone.0087888

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© 2014 Kelly et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Reciprocal grafting of WT, AQP1 and HK4 plants was performed at the seedling stage. The plants were photographed (A and Fig. 2A in [30]) and their transpiration was measured about 4 weeks after grafting. (B) Whole-plant relative daily transpiration of reciprocal-grafted plants. Data were normalized to the total plant weight and the amount of water taken up by the neighboring submerged fixed-size wick each day, which was set to 100%. Presented data are means of four independent repeats ± SE. Different letters indicate a significant difference (t test, P<0.05).