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. 2014 Feb 3;9(2):e87887. doi: 10.1371/journal.pone.0087887

Figure 1. Exposure and immunization schedule.

Figure 1

To make long-term dermal exposure, male Balb/c mice were smeared with 0.1 mL saline or DBP (0.4, 4.0 and 4.0 mg/(kg•d)) from day 1 to 40 (40 times) on their shaven backs, and then sensitized with 120 µL of saline or 0.5% FITC (in 1∶1 acetone/DBP) on days 41 and 42, on their shaven backs. On day 47, baseline ear thickness was measured with calipers followed by challenge with 20 µL of saline or 0.5% FITC on the right ear, and saline or vehicle (1∶1 DBP/acetone) on the left ear. n = 8 mice in each group. (1) Saline, (2) DBP 0.4, (3) DBP 4, (4) DBP 40, (5) FITC, (6) FITC+DBP 0.4, (7) FITC+DBP 4, (8) FITC+DBP 40.