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. 2014 Feb 3;9(2):e87811. doi: 10.1371/journal.pone.0087811

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© 2014 Dornas et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Purified APMV (10⁶ viral particles) were added to VD samples, and subsequently, BAL samples were added. The BAL-VD samples were or were not enriched and maintained at room temperature. The samples were titrated in amoebae monthly. (A) Samples without enrichment; (B) Enriched samples. The results are the means+SD of an experiment performed in quintuplicate.