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Purified APMV (106 viral particles) was added to salt water, soil, fresh water and VD substrates, which were then enriched. After viral isolation from each substrate, A. castellanii were infected at an MOI of 10. The infectivity was measured by TCID50 after 25 hours (0 to 25 hours) by observing CPE in amoeba. The results are the means of experiments performed in duplicate.
