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Messenger RNA levels of ERα were measured by QT-PCR from (A) 2 and 6 months-old male Wistar rats after 72 h glucose and intralipid co-infusion.(B) C57bl/6J male mice of 8–12 weeks-old after a 4 day glucose infusion. (C) Wistar rat islets cultured for one week in glucose 5, 10 or 30 mM. (D) β-cells cell line from control Lox/Lox and βIRKO mice cultured in 16.7 or 33 mM glucose. Values represent the mean±SEM, n = 4–5/group. *P<0.05, **P<0.01, ***P<0.001.
