Figure 2. FXR is required for CDCA-induced activation of the −278/+10 SHP promoter.

DLD-1 cells were transfected with hFXR and hRXRα expression plasmids and various hSHP promoter constructs as indicated. Cells were treated with or without 100 µmol/L CDCA. Luciferase activity was measured to determine the SHP promoter activity. Data are presented as means ± SD; n≥3. P≤0.05 for *) in a pairwise comparison by Mann-Whitney U test.