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. 2014 Feb 3;9(2):e88011. doi: 10.1371/journal.pone.0088011

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© 2014 Hoeke et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) shows an overview of the different constructs used to localize the FXR-responsive element in the −278/−69 region of the SHP promoter. Relevant binding sites for other NRs are included. (B, C) DLD-1 cells were transfected with the indicated hSHP promoter constructs and expression plasmids for hFXR and hRXRα. Cells were treated with or without 100 µmol/L CDCA (B) or 1 µmol/L GW4064 (C). Luciferase activity was measured to determine SHP promoter activity. Data are presented as means of ± SD; n≥3. Significant differences are indicated when compared to CDCA/GW4064-treated −569/+10 (a); CDCA/GW4064-treated −303/+10 (b); CDCA/GW4064-treated −278/+10(c). P≤0.05 in a pairwise comparison by Mann-Whitney U test.