Skip to main content
. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cell Mol Bioeng. 2013 Jul 19;6(3):326–334. doi: 10.1007/s12195-013-0291-x

Figure 3.

Figure 3

Effect of shear stress on infectivity and viability of monocytes. THP1 monocytes were infected with mock (only media) or Chlamydia pneumoniae at MOI 2 for 36 h. The cells were resuspended in fresh media, and sheared at 0 (static) or 7.5 dyn/cm2 (shear) for 1 h. (a) The cells were incubated for an additional 32 h to finish 72 h of infection cycle and then fixed and stained with anti-Chlamydia pneumoniae antibody and DAPI. Chlamydial inclusions (green) and THP1 nucleus (blue) are shown. Scale bar = 20 μM. (b) The viability was assessed by trypan blue exclusion. The results are mean ± SD of one representative experiment performed in triplicate, and the experiments were repeated three times.