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. 2014 Feb 3;9(2):e87976. doi: 10.1371/journal.pone.0087976

Table 1. detection of nif genes in metagenomic sequencing of acid mine drainage.

Gene Function sequence number
nifH nitrogenase reductase 82
nifD nitrogenase molybdenum-iron protein subunit alpha 127
nifK nitrogenase molybdenum-iron protein subunit beta 93
nifE nitrogenase molybdenum-cofactor biosynthesis protein 85
nifN nitrogenase molybdenum-cofactor biosynthesis protein 75
nifX iron-molybdenum cofactor processing protein 26
nifA Nif-specific regulatory protein 89
nifB FeMo cofactor biosynthesis protein 78
nifQ molybdenum ion binding protein 1
nifS cysteine desulfurase 11
nifT nitrogen fixation protein 0
nifU Fe-S cluster assembly protein 2
nifV homocitrate synthase 62
nifW nitrogenase stabilizing/protective protein 2
nifZ iron-sulfur cofactor synthesis protein 1
nifJ pyruvate-flavodoxin oxidoreductase 6
nifL nitrogen fixation negative regulator 1
nifP serine acetyltransferase 1
total 742