Table 2. List of ORFs from fosmid DX-1A-14, gene length, and similar genes in GenBank.
ORF | Gene length (bp) | Close relative (protein, [organism], identitya) |
1 | 594 | Nitrogenase MoFe protein [Herbaspirillum seropedicae SmR1] 80% |
2 | 1515 | MoFe cofactor biosynthesis protein NifE [Burkholderia vietnamiensis G4] 79% |
3 | 1362 | MoFe cofactor biosynthesis protein NifN [Herbaspirillum seropedicae SmR1] 68% |
4 | 405 | MoFe cofactor biosynthesis protein NifX [Herbaspirillum seropedicae SmR1] 74% |
5 | 462 | Hypothetical protein [Herbaspirillum seropedicae SmR1] 71% |
6 | 204 | Hypothetical protein [Beijerinckia indica subsp. indica ATCC 9039] 58% |
7 | 315 | 4Fe-4S ferredoxin [Rubrivivax benzoatilyticus JA2] 70% |
8 | 576 | NifQ family protein [Candidatus Accumulibacter phosphatis clade IIA] 52% |
9 | 294 | Ferredoxin protein [Herbaspirillum seropedicae SmR1] 74% |
10 | 1299 | Ferredoxin protein [Herbaspirillum seropedicae SmR1] 79% |
11 | 1089 | Ferredoxin protein [Herbaspirillum seropedicae SmR1] 80% |
12 | 849 | Ferredoxin protein [Herbaspirillum seropedicae SmR1] 80% |
13 | 363 | Nitrogenase stabilizing protein [Herbaspirillum seropedicae SmR1] 50% |
14 | 1131 | Homocitrate synthase [Herbaspirillum seropedicae SmR1] 63% |
15 | 1587 | Transmembrane protein [Ralstonia eutropha H16] 59% |
16 | 240 | Conserved hypothetical protein [Methylococcus capsulatus str. Bath] 55% |
17 | 1659 | Nif-specific regulatory protein [Herbaspirillum seropedicae SmR1] 61% |
18 | 1872 | Fe-S protein assembly chaperone HscA [Methylovorus sp. SIP3-4] 69% |
19 | 357 | Iron-sulfur cluster insertion protein ErpA [Nitrosomonas europaea] 69% |
20 | 1563 | FeMo cofactor biosynthesis protein [Herbaspirillum seropedicae SmR1] 83% |
21 | 753 | Conserved hypothetical protein [Herbaspirillum seropedicae SmR1] 60% |
22 | 240 | Putative NifZ protein [Methylococcus capsulatus str. Bath] 60% |
23 | 1161 | Aminotransferase class V [Beijerinckia indica subsp. indica ATCC 9039] 58% |
24 | 267 | Conserved hypothetical protein [Rhodopseudomonas palustris BisB18] 43% |
25 | 1629 | Rhodanese domain protein [Beijerinckia indica subsp. indica ATCC 9039] 55% |
26 | 414 | Oxygen-binding (globin) protein [Herbaspirillum seropedicae SmR1] 68% |
27 | 426 | Two component regulator protein [Herbaspirillum seropedicae SmR1] 60% |
28 | 4557 | Methyl-accepting chemotaxis transducer [Acidovorax delafieldii 2AN] 51% |
29 | 990 | RuBisCO operon transcriptional regulator [Beggiatoa sp. PS] 54% |
30 | 1422 | RubisCO formI large subunit [Acidithiomicrobium sp.] 91% |
31 | 333 | RubsiCO small subunit [Acidithiobacillus ferrivorans SS3] 70% |
32 | 2382 | Carboxysome shell polypeptide [Halothiobacillus neapolitanus c2] 46% |
33 | 1497 | Carboxysome shell carbonic anhydrase [Halothiobacillus neapolitanus c2] 58% |
Nucleotide identity of fosmid DX-1A-14 gene to the gene of the organism to which it is most related.