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Southern dot blot showing (I) SCOTS clones (each dot corresponds to one clone in Table 2) and (II) 0.1 µg (top) or 1 µg (bottom) of genomic S. agalactiae DNA as positive controls, hybridized to a digoxigenin (DIG)-labeled probe generated from RAW264.7-derived (a) or culture-derived cDNA (b) after three rounds of normalization.
