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. 2014 Feb 3;9(2):e87980. doi: 10.1371/journal.pone.0087980

Figure 9. qRT-PCR determination of the genes up-regulated during intracellular survival of S. agalactiae.

Figure 9

Real-time RT-PCR analysis of differentially expressed genes of S. agalactiae vis a vis survival in RAW264.7 and in THB cultures. Twelve genes, aroA, clone B3 (peptidase), clone A6 (polysaccharide biosynthesis protein), clone E4 (oligopeptide ABC transporter oligopeptide -binding protein), covR, divIB, dltA, dnaJ, hylB, pulA, secA and serB were selected and amplified using real-time RT-PCR. Real time RT-PCR data for each gene is relative to that obtained for the 16S rRNA control. Data points represent the means ± SD of three independent experiments.