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. 2014 Feb 3;9(2):e87980. doi: 10.1371/journal.pone.0087980

Table 2. Genes identified by SCOTS that are differentially expressed by S. agalactiae upon interaction with RAW264.7.

Function and clone Gene and/or possible function GenBank Identification Reference(s)
Cell envelope biogenesis, outer membrane
Clone A1 cfa, YP_330368.1
cyclopropane-fatty-acyl-phospholipid synthase (S.agalactiae A909)
Clone A2 dltA, ZP_08649940.1 [29], [30], [31], [32], [33], [34]
D-alanine-D-alanyl carrier protein ligase subunit 1 (S.agalactiae ATCC 13813)
Clone A3 D-alanyl-D-alanine carboxypeptidase (S. agalactiae 2603V/R) NP_687183.1
Clone A4 cell wall surface anchor family protein (S.agalactiae A909) YP_329518.1
Clone A5 secA, ZP_08650039.1 [35], [36]
preprotein translocase subunit SecA (S.agalactiae ATCC 13813)
Clone A6 polysaccharide biosynthesis protein (S.agalactiae A909) YP_330055.1
Clone A7 cstA, NP_735499.1
hypothetical protein gbs1050 (S. agalactiae NEM316)
Clone A8 hypothetical protein FSLSAGS3026_12085 (S. agalactiae FSL S3-026) EGS27102.1
Clone A9 Can B domain-containing protein (S.agalactiae FSL S3-026) EGS27960.1
Metabolism/stress response
Clone A10 hypothetical protein SAG0894 (S. agalactiae 2603V/R) NP_687908.1
Clone B1 hylB, YP_329897.1 [9]
hyaluronate lyase (S. agalactiae A909)
Clone B2 clpE, NP_734999.1 [40], [41]
hypothetical protein gbs0535 (S. agalactiae NEM316)
Clone B3 peptidase, M20/M25/M40 family (S. agalactiae H36B) ZP_00784166.1
Clone B4 metallopeptidase, zinc binding (S. agalactiae COH1) ZP_00784711.1
Clone B5 endopeptidase O, putative (S. agalactiae CJB111) ZP_00788097.1
Clone B6 hypothetical protein SAG0872 (S. agalactiae 2603V/R) NP_687886.1
Clone B7 pulA, ZP_00786133.1 [46], [47], [48], [49], [50]
pullulanase, putative (S. agalactiae COH1)
Clone B8 mannosyl-glycoprotein endo-beta-N-acetylglucosamidase family protein (S. agalactiae FSL S3-026) EGS28355.1
Clone B9 ciaH, NP_735468.1 [37], [38], [39]
sensor histidine kinase CiaH (S. agalactiae NEM316)
Clone B10 serB, NP_687649.1
phosphoserine phosphatase SerB (S. agalactiae 2603V/R)
Clone C1 acetyltransferase (S. agalactiae 2603V/R) NP_688337.1
Clone C2 pyridine nucleotide-disulfide oxidoreductase family protein (S. agalactiae 2603V/R) NP_688351.1
Clone C3 htpX, NP_688618.1
heat shock protein HtpX (S. agalactiae 2603V/R)
Clone C4 CsbD family protein (S. agalactiae A909) YP_329332.1
Clone C5 fhs, YP_329765.1
formate—tetrahydrofolate ligase (S. agalactiae A909)
Clone C6 folP, YP_329821.1
dihydropteroate synthase (S. agalactiae A909)
Clone C7 aroA, ZP_08650982.1
3-phosphoshikimate-1-carboxyvinyltransferase (S. agalactiae ATCC 13813)
Clone C8 nox, ZP_00783526.1 [42]
NADH oxidase (S. agalactiae H36B)
Clone C9 morphine 6-dehydrogenase (S. agalactiae ATCC 13813) ZP_08650234.1
Clone C10 carB, ZP_08650601.1 [43]
carbamoyl-phosphate synthase, large subunit (S. agalactiae ATCC 13813)
Clone D1 msrB, ZP_00785672.1 [44], [45]
methionine sulfoxide reductases B (S. agalactiae COH1)
Clone D2 nrdI, YP_330497.1
flavoprotein NrdI (S. agalactiae A909)
Clone D3 pyrH, NP_688507.1
uridylate kinase (S. agalactiae 2603V/R)
Clone D4 sdhA, YP_330694.1
L-serine dehydratase, iron-sulfur-dependent subunit alpha (S. agalactiae A909)
Clone D5 aroD, 3-dehydroquinate dehydratase (S. agalactiae 2603V/R) NP_688377.1
Clone D6 hypothetical protein SAK_0322 (S. agalactiae A909) YP_328974.1
Clone D7 fabK, NP_687380.1
enoyl-ACP reductase (S. agalactiae 2603V/R)
Clone D8 cylI, EGS28236.1
cylI protein (S. agalactiae FSL S3-026)
Clone D9 gap, NP_688758.1 [51], [52]
glyceraldehyde-3-phosphate dehydrogenase (S. agalactiae 2603V/R)
Clone D10 glycosyl hydrolase (S. agalactiae A909) YP_329453.1
Clone E1 ccpA, YP_329455.1 [53]
catabolite control protein A (S. agalactiae A909)
Transport
Clone E2 voltage-gated chloride channel family protein (S. agalactiae 2603V/R) NP_687570.1
Clone E3 ABC transporter ATP-binding protein (S. agalactiae 2603V/R) NP_689021.1
Clone E4 oligopeptide ABC transporter oligopeptide-binding protein (S. agalactiae 2603V/R) NP_687222.1
Clone E5 thiW protein (S. agalactiae ATCC 13813) ZP_08650771.1
Cell division/replication
Clone E6 divIB, NP_687507.1
cell division protein DivIB (S. agalactiae 2603V/R)
Clone E7 uvrA, ZP_00783998.1
excinuclease ABC, A subunit (S. agalactiae H36B)
Clone E8 MutS2 family protein (S. agalactiae FSL S3-026) EGS26693.1
Clone E9 IS861, transposase OrfB (S. agalactiae 2603V/R) NP_688077.1
Clone E10 rpoC, EGS28475.1
DNA-directed RNA polymerase subunit beta (S. agalactiae FSL S3-026)
Protein sorting
Clone F1 dnaJ, ZP_00783391.1
dnaJ protein (S. agalactiae H36B)
Clone F2 lepB, ZP_00788470.1
Signal peptidase I (S. agalactiae CJB111)
Regulatory
Clone F3 hrcA, YP_328819.1
heat-inducible transcription repressor (S. agalactiae A909)
Clone F4 MerR family transcriptional regulator (S. agalactiae A909) YP_330526.1 [54]
Clone F5 LysR family transcriptional regulator (S. agalactiae NEM316) NP_735874.1 [55]
Clone F6 covR, YP_004478477.1 [10]
response regulator protein (S. parauberis KCTC 11537)
Unknown function
Clone F7 unknown (S. agalactiae H36B) ZP_00783887.1
Clone F8 hypothetical protein SAG1491 (S. agalactiae 2603V/R) NP_688485.1
Clone F9 yobC, AAL96904.1
hypothetical protein spyM18_0084 (S.pyogenes MGAS8232)
Clone F10 hypothetical protein FSLSAGS3026_02113 (S.agalactiae FSL S3-026) EGS28676.1