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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Biomed Mater Res A. 2013 Jun 11;102(5):1361–1369. doi: 10.1002/jbm.a.34814

Figure 1.

Figure 1

Device set-up and cell alignment scheme. (A) Wells mounted in Hoffman clamps are housed in square petri dishes, containing an open petri dish (round) of water for humidification. (B) Plasma oxidized wells are coated with silane that serves as a binding site for Pluronic blocking agents. The passivated well is filled with extracellular matrix (ECM) protein solution and mounted in a Hoffman clamp. Cracks form in the oxidized surface layer when pressure is applied by tightening the screw clamp, and protein fills the cracks, presenting an adhesive surface for cells.