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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Biomed Mater Res A. 2013 Jun 11;102(5):1361–1369. doi: 10.1002/jbm.a.34814

Figure 4.

Figure 4

Crack spacing, protein adherence, and staining of aligned cells. Cracks were visualized with TRITC-BSA (red), and the average spacing resulting from (A) 10% and (B) 20% compressive loads was respectively 39.5 ± 19.8 μm and 19.0 ± 9.1 μm (mean ± S.D.). Data was collected from five different fields in the well region of each of three cuboids for each compressive load. Scale bar = 25 μm (C) Phase-contrast image of N27 cells aligned on cracks. (D) Aligned N27 cells were counterstained with FITC-labeled Phalloidin (red) and DAPI (blue, to label nuclei) revealing aligned actin filaments and elongated nuclei. Scale Bar = 50 μm.