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. 2014 Feb 3;9(2):e86786. doi: 10.1371/journal.pone.0086786

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© 2014 Noujaim et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Bar graph showing the normalized Histone H3 band intensity in P radiograms at 3 different microtubule (MT) concentrations. (B) Image of a P radiogram showing radioactive bands corresponding to phosphorylated INBox motif (labeled) and Histone H3 (labeled) at 2 different microtubule concentrations. (C) Bar graph showing the normalized INBox band intensity in P radiograms at 3 different microtubule (MT) concentrations. (D) Image of an SDS-PAGE gel showing bands corresponding to Aurora-B-GFP (labeled), tubulin (labeled), and Histone H3 (labeled) at 2 different microtubule concentrations. The band for the INBox construct, at 52 kDa, is masked by the tubulin band. (E) Bar graph showing the normalized MCAK^AAA band intensity in P radiograms at 3 different microtubule (MT) concentrations. (F) Image of a P radiogram showing radioactive bands corresponding to phosphorylated MCAK^AAA (labeled) and the INBox motif (labeled) at 2 different microtubule concentrations.

Inline graphic — (A) Bar graph showing the normalized Histone H3 band intensity in P radiograms at 3 different microtubule (MT) concentrations. (B) Image of a P radiogram showing radioactive bands corresponding to phosphorylated INBox motif (labeled) and Histone H3 (labeled) at 2 different microtubule concentrations. (C) Bar graph showing the normalized INBox band intensity in P radiograms at 3 different microtubule (MT) concentrations. (D) Image of an SDS-PAGE gel showing bands corresponding to Aurora-B-GFP (labeled), tubulin (labeled), and Histone H3 (labeled) at 2 different microtubule concentrations. The band for the INBox construct, at 52 kDa, is masked by the tubulin band. (E) Bar graph showing the normalized MCAK^AAA band intensity in P radiograms at 3 different microtubule (MT) concentrations. (F) Image of a P radiogram showing radioactive bands corresponding to phosphorylated MCAK^AAA (labeled) and the INBox motif (labeled) at 2 different microtubule concentrations.