A. Western blotting for p21 in the nuclear fraction (nP21) and p21 in the cytoplasmic fraction (cP21) from Huh7 stable cell lines with altered 15-PGDH expression. Histone and β-actin were used as the internal control, respectively.
B. Co-immunoprecipitation and western blotting analysis using indicated antibodies in Huh7 stable cell lines with altered 15-PGDH expression. IgG IP was used as the negative control. PCNA western blotting was used as input control.
C. Co-immunoprecipitation and western blotting analysis using indicated antibodies in Huh7 stable cell lines with altered 15-PGDH expression. IgG IP was used as the negative control. rIP denotes repeat co-immunoprecipitation.
D. Co-immunoprecipitation and western blotting analysis using indicated antibodies in Huh7 stable cell lines with altered 15-PGDH expression. IgG IP was used as the negative control. rIP denotes repeat co-immunoprecipitation.
E. Co-immunoprecipitation and western blotting analysis using indicated antibodies in Huh7 stable cell lines with altered 15-PGDH expression. IgG IP was used as the negative control.