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. Author manuscript; available in PMC: 2014 Aug 27.
Published in final edited form as: Oncogene. 2013 Apr 1;33(9):1101–1112. doi: 10.1038/onc.2013.69

Figure 6. The effect of 15-PGDH signaling on p21 downstream molecules in HCC cells.

Figure 6

A. Western blotting for p21 in the nuclear fraction (nP21) and p21 in the cytoplasmic fraction (cP21) from Huh7 stable cell lines with altered 15-PGDH expression. Histone and β-actin were used as the internal control, respectively.

B. Co-immunoprecipitation and western blotting analysis using indicated antibodies in Huh7 stable cell lines with altered 15-PGDH expression. IgG IP was used as the negative control. PCNA western blotting was used as input control.

C. Co-immunoprecipitation and western blotting analysis using indicated antibodies in Huh7 stable cell lines with altered 15-PGDH expression. IgG IP was used as the negative control. rIP denotes repeat co-immunoprecipitation.

D. Co-immunoprecipitation and western blotting analysis using indicated antibodies in Huh7 stable cell lines with altered 15-PGDH expression. IgG IP was used as the negative control. rIP denotes repeat co-immunoprecipitation.

E. Co-immunoprecipitation and western blotting analysis using indicated antibodies in Huh7 stable cell lines with altered 15-PGDH expression. IgG IP was used as the negative control.