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Rapid tissue clearing with ClearT. (A) Fixed whole embryos (E14.5) and dissected postnatal brains (P0) were cleared overnight. The grid is visible through tissue cleared by ClearT. (B) E14.5 embryos cleared with ClearT or ScaleA2 reach full transparency in 1 day or 14 days, respectively. (C) ClearT does not lead to volume changes. P0 sections (800 μm), surface area measured: pre-cleared, red line; ClearT, blue line. (D) Clearing is reversible with PBS (30 minutes). Scale bars: 1 mm.
