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. 2012 Dec 15;139(24):4514–4523. doi: 10.1242/dev.083279

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© 2012. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 1. — Cellular localization of LIN28 protein in fully grown mouse CD1 oocytes. Confocal immunofluorescence analysis of endogenous LIN28 in whole-mount oocytes. B23 (NPM) was used as a nucleolar marker. DNA was counterstained with DAPI. In NSN oocytes (A-D) LIN28 and NPM localize at the periphery of the nucleolus. During transition from NSN to SN configuration, both LIN28 and NPM gradually disappear from surface of the nucleolus and they are not detected there in SN oocytes (E-H). Insets show higher magnification of nucleus. Scale bar: 20 μm.

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