Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Dec 15;139(24):4514–4523. doi: 10.1242/dev.083279

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

PMC Copyright notice

Fig. 2. — Cellular localization of LIN28 protein during mouse preimplantation development. Confocal immunofluorescence analysis of endogenous LIN28 in whole-mount embryos. B23 (NPM) was used as a nucleolar marker. DNA was counterstained with DAPI. An enlarged view of the boxed regions show that LIN28 (green) colocalizes with NPM (red). (A-D) In zygotes, homogenous nuclear signals of both LIN28 and NPM were observed. No enrichment at the periphery of nucleolar precursor bodies (NPBs) was observed for either marker. (E-P) At the early and mid 2-cell stage, LIN28 localizes at the periphery of nucleolar precursor bodies (NPBs) (arrow), where it colocalizes with NPM (E-H). LIN28 remains colocalized with NPM at NPBs (arrow) at the 4-cell (I-L) and 8-cell (M-P) stages. (Q-T) As NPBs have transformed to mature nucleoli in morula embryos, LIN28 remains nucleolar, as confirmed by the colocalization with NPM. (U-X) At the blastocyst stage, the localization of LIN28 in the nucleolus is concordant with NPM. LIN28 was present in both TE and ICM cells. Scale bar: 20 μm.

HHS Vulnerability Disclosure