Fig. 5.

Cellular localization of LIN28 protein in early embryos and ESCs of the common marmoset monkey. Confocal immunofluorescence analysis of endogenous LIN28. B23 (NPM) was used as a nucleolar marker. DNA was counterstained with DAPI. (A-D) In 11-cell embryos, LIN28 is enriched in the nucleoplasm and not at the periphery of NPBs, whereas NPM is already present at the periphery of NPBs. An enlarged view of the boxed region shows that LIN28 (green) does not colocalize with NPM (red). (E-H) In blastocyst embryos, LIN28 colocalizes with NPM at the nucleolus, but only in trophoblast (TE) and not ICM cells. ICM cells only show a NPM signal (red). Boxed region in H shows full projection of ICM and TE cells, which are displayed as z-sections in supplementary material Fig. S5. (I-L) In ESCs, LIN28 is predominantly cytoplasmic and absent from the nucleolus. Scale bars: 20 μm.