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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Nat Med. 2013 Dec 15;20(1):54–61. doi: 10.1038/nm.3423

Figure 4. HFD increases NLRP3, which is required for AHR.

Figure 4

a. Total RNA was extracted from the lung, liver and adipose tissue and assessed for Nlrp3. Fold induction of Nlrp3 mRNA was calculated based on GAPDH expression. **p<0.01 and ***p<0.001, mRNA level from mice fed on HFD compared to chow group. (Student’s t-test)

b. Graph represent grams of weight gain in WT or Nlrp3−/− mice. **p<0.01, WT mice on HFD compared to Nlrp3−/− mice on HFD group. (Student’s t-test)

c. WT and Nlrp3−/− mice 3 months after HFD (left). Representative pictures of lung, liver and epididymal adipose tissue from Nlrp3−/− mice fed on chow or HFD (right). Scale bar indicates 5mm

d. Development of obesity induced AHR was compared in WT and Nlrp3−/− mice. Graph represents the changes in lung resistance (RL). *p<0.05, Nlrp3−/− mice on HFD were compared to WT mice on HFD group (Two-way ANOVA).

e. IL-1β levels in the culture supernatant of lung cells from WT and Nlrp3−/− mice. The data shown are means ± SEM. **p<0.01, supernatants from HFD group were compared to chow group. (Student’s t-test)

f. Lungs were taken from chow or HFD fed mice and stimulated with PMA/ionomycin for 5 hr. −IL-17A+ ILC3 cells in WT mice (upper panel) or Nlrp3−/− mice (lower panel) were assessed by FACS. Graph represents the total number of ILC3 cells in the lung. ***p<0.001, ILC3 cells in WT mice were compared to Nlrp3−/− mice. (Student’s t-test).