Figure 5.

Endocytosis of the A-2F in human pancreatic tumor cells (MIA PaCa-2). 3 µmol/l A-2F-ATTO 633 was incubated in cultured cells. (a) After incubation, fluorescence signal was mainly present on plasma membrane. (b) Significant vesicular signals could be observed into cell cytoplasm after 60 minutes since incubation, but A-2F fluorescence was still present on the cell surface. (c) At longer times (140 minutes) all A-2F was internalized into the cells and perinuclear signals were observed. Scale bars: 10 μm.