Table 7.
Levels of miR-34a and miR-34c repression of four target genes' expression.
| miR-34a vs. scrambled | miR-34c vs. scrambled | miR-34a + 34c vs. scrambled | |
|---|---|---|---|
| Protein | Mean % repression (SD) | Mean % repression (SD) | Mean % repression (SD) |
| Onecut2 | 27.15 (3.34) | 40.13 (2.85) | 46.42 (0.63) |
| SIRT1 | 22.28 (7.68) | 26.09 (6.78) | 34.89 (5.92) |
| Bcl2 | 23.27 (2.87) | 16.06 (2.48) | 32.57 (1.85) |
| Psen1 | 10.69 (4.8) | 18.89 (5.94) | 25.54 (6.39) |
| β-actin | 0 | 0 | 0 |
Percent repression of target proteins in HEK 293 cells transfected with miR-34a, miR-34c, or both miRNAs. The repression levels shown here represent protein band intensities, after normalization against β-actin band intensities, when cultures transfected by microRNA are compared with those transfected by scrambled controls. As shown in Figure 8, the % change of β-actin levels between cultures transfected by the microRNAs of interest and by the scrambled controls is ~0. Mean % repression with standard deviation (SD) of each miR for each protein is tabulated.