Fig. 6.

a Dose-dependent induction of NF-AT/GFP reporter. An RBL-703/21 clone sorted by FACS stably transfected with NF-AT-hrGFP was sensitised overnight with 1:40 diluted (v/v) serum of an allergic patient. FN-bound cells were stimulated with varying dilutions of Bet v1 (black symbols) or Phl p1 (open symbols) in two separate experiments (expt. 1: triangles; expt. 2: circles). Data shown are mean fluorescence ± SD from triplicate determinations. b Negative and positive control experiments for the experiments are shown in Fig. 6a. The mean ± SD from triplicate determinations of the RBL NF-AT-GFP reporter unsensitised and unstimulated (cell only), sensitised with IgE and unstimulated (IgE only), sensitised with IgE and stimulated with anti-IgE (IgE+anti-IgE), sensitised with serum and unstimulated (serum only) or sensitised with serum and stimulated with anti-IgE (serum+anti-IgE) are shown. **p < 0.01, paired Student’s t test. n.s. not significant. c Fluorescent microscope image (bar: 0.4 mm) of IgE/anti-IgE-stimulated RBL NF-AT-GFP reporter cells