Fig. 8.

a Dose-dependent induction of NF-AT/DsRed reporter. An RBL-703/21 clone sorted by FACS stably transfected with NF-AT-DsRed was sensitised overnight with 1:20 (v/v) diluted pooled serum from ten allergic patients monosensitised to Parietaria judaica. FN-bound cells were stimulated with varying dilutions of Par j 2 (black circles expt.1, black triangles expt. 2). The graph also shows the lack of DsRed induction in cells which were not sensitised with serum in the second experiments (to control for autofluorescence of the used allergen). Data shown are mean fluorescence ± SD from triplicate determinations. b Negative and positive control experiments for the experiments shown in Fig. 6a. The mean ± SD from triplicate determinations of the RBL NF-AT-DsRed reporter unsensitised and unstimulated (cell only), sensitised with IgE and unstimulated (IgE only), sensitised with IgE and stimulated with anti-IgE (IgE+anti-IgE), sensitised with serum and unstimulated (serum only) or sensitised with serum and stimulated with anti-IgE (serum+anti-IgE) are shown. ***p < 0.001, paired Student’s t test. n.s. not significant. c Fluorescent microscope image (bar: 0.4 mm) of IgE/anti-IgE-stimulated RBL NF-AT-DsRed reporter cells