Figure 1.

PAMP tiling design for capture of CDKN2A deletions. CDKN2A upstream and downstream breakpoint regions are defined on a germline genome, blue and red lines, respectively. Tiled forward primers (blue arrows) and reverse primers (red arrows) are spaced ≈1 kb apart (width of hashed boxes; not to scale with reference). Overlap of blue box and red box on tumor DNA indicates that a forward and reverse primer pair is <2 kb apart and will lead to amplification of tumor DNA harboring CDKN2A deletion breakpoints.