Figure 9.

cAMP responsive element binding protein phosphorylation was significant reduced after repetitive mTBI. Wild-type C57BL/6J mice (male, 3 months of age) received three mild impacts (mTBI) or underwent sham procedures (sham). Mice were sacrificed 6 months later and brains were fixed for immunohistochemistry with an antibody against p-CREB. Representative images were taken from amygdala (A) and hippocampus (C), and p-CREB immunoreactivity (B,D) was quantified as percentage of area occupied. Scale bar in (A,C) = 20 μm. n = 8 (sham) and 15 (mTBI) mice/group. Mean ± SEM. *P < 0.05, by t test.