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. 2014 Feb 3;204(3):331–342. doi: 10.1083/jcb.201310136

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© 2014 Iijima et al.

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Figure 6. — Alternative splicing defects in SLM1^KO mice. (A) Incorporation of alternative exons at AS3, -4, and -5 in midbrain cDNA samples was probed by semi-quantitative PCR with primers flanking the insertion site and analyzed by gel electrophoresis. (B) Representative images of semi-quantitative RT-PCR with Nrxn AS4 performed on cortex, midbrain, and brainstem from wild-type, Slm1^KO, Sam68:Slm1^DKO, and Sam68^KO mice. (C) Quantitative RT-PCR performed on cortex and midbrain cDNA samples from wild-type, Slm1^KO, Sam68:Slm1^DKO, and Sam68^KO mice (n = 3 animals per genotype).