Table 1.
Strains and plasmids used in this paper
| Strain or plasmid name | Genotype | Reference |
| AB33nRFP | a2 PnarbW2 PnarbE1, bleR/poNLS3RFP | Schuster et al., 2011b |
| AB33 | a2 PnarbW2 PnarbE1, bleR | Brachmann et al., 2001 |
| AB33R25G | a2 PnarbW2 PnarbE1, Prpl25-rpl25-egfp, bleR, hygR | This paper |
| AB33R3Ch3 | a2 PnarbW2 PnarbE1, Prps3-rps3-3xmcherry, bleR, hygR | This paper |
| AB33R25paG | a2 PnarbW2 PnarbE1, Prpl25-rpl25-pagfp, bleR, hygR | This paper |
| AB33paG2 | a2 PnarbW2 PnarbE1, bleR/popaG2 | This paper |
| AB33R3Ch3_R25G | a2 PnarbW2 PnarbE1, Prps3-rps3-3xmcherry, Prpl25-rpl25-egfp, bleR, hygR, natR | This paper |
| AB33ΔKin3_R25G | a2 PnarbW2 PnarbE1, Δkin3, Prpl25-rpl25-egfp, bleR, natR, hygR | This paper |
| AB5Dyn2ts_R25G | a1 PnarbW2 PnarbE1, Pdyn2-Dyn2ts, Prpl25-rpl25-egfp, bleR, hygR, natR | This paper |
| AB33ΔKin3_Kin3ts_R25G | a2 PnarbW2 PnarbE1, Δkin3, Prpl25-rpl25-egfp, bleR, natR, cbxR/pKin3ts | This paper |
| AB33R25G_ChRab5a_ΔRrm4 | a2 PnarbW2 PnarbE1, Prpl25-rpl25-egfp, Δrrm4, bleR, hygR, cbxR/pomChRab5a | This paper |
| AB33EG | a2 PnarbW2 PnarbE1, bleR/pERGFP | Wedlich-Söldner et al., 2002a |
| AB33ΔKin3_EG_Kin3ts | a2 PnarbW2 PnarbE1, Δkin3, bleR, natR/pERGFP/pKin3ts | This paper |
| AB5Dyn2ts_EG | a1 PnarbW2 PnarbE1, Pdyn2-Dyn2ts, bleR, hygR/pERGFP | This paper |
| AB33R25G_ChRab5a | a2 PnarbW2 PnarbE1, Prpl25-rpl25-egfp, bleR, hygR/pomChRab5a | This paper |
| AB33GRab5a | a2 PnarbW2 PnarbE1, bleR/poGRab5a | Schuster et al., 2011a |
| AB33ChRab5a_PXG | a2 PnarbW2 PnarbE1, bleR/pomChRab5a/poPXG | This paper |
| AB33ChRab5a_GRab4 | a2 PnarbW2 PnarbE1, bleR/pomChRab5a/poGRab4 | This paper |
| AB33ChRab5a_GRab7 | a2 PnarbW2 PnarbE1, bleR/pomChRab5a/poGRab7 | This paper |
| AB33ΔKin3 | a2 PnarbW2 PnarbE1, Δkin3, bleR, natR | This paper |
| FB2N107G | a2 b2 Pnup107-nup107-egfp, bleR | Steinberg et al., 2012 |
| AB33ChRab5a_G3Rho3 | a2 PnarbW2 PnarbE1, bleR/pomChRab5a/poG3Rho3 | This paper |
| AB33Rrm4G | a2 PnarbW2 PnarbE1, Prrm4-rrm4-egfp, bleR, hygR | This paper |
| AB33ChRab5a_Rrm4G | a2 PnarbW2 PnarbE1, Prrm4-rrm4-egfp, bleR, hygR/pomChRab5a | This paper |
| AB33R3Ch3_Rrm4G | a2 PnarbW2 PnarbE1, Prps3-rps3-3xmcherry, Prrm4-rrm4-egfp, bleR, natR, hygR | This paper |
| AB33ΔKin3_Kin3ts_GRab5a | a2 PnarbW2 PnarbE1, Δkin3, bleR, natR/pKin3ts/pCoGRab5a | This paper |
| AB5Dyn2ts_GRab5a | a1 PnarbW2 PnarbE1, Pdyn2-Dyn2ts, bleR, hygR/poGRab5a | Schuster et al., 2011c |
| AB33GRab7 | a2 PnarbW2 PnarbE1, bleR/poGRab7 | This paper |
| AB33Yup1ts_GRab5a | a2 PnarbW2 PnarbE1, Pyup1-yup1ts, bleR, hygR, cbxR/poGRab5a | This paper |
| AB33Yup1ts_R25G | a2 PnarbW2 PnarbE1, Pyup1-yup1ts, Prpl25-rpl25-egfp, bleR, hygR, cbxR | This paper |
| FB2N107R_cG | a2 b2 Pnup107-nup107-mrfp, bleR/pcrgG | This paper |
| AB33R3G | a2 PnarbW2 PnarbE1, Prps3-rps3-egfp, bleR, hygR | This paper |
| AB33G3Dyn2 | a2 PnarbW2 PnarbE1, Pdyn2-3×egfp-dyn2, bleR, hygR | Lenz et al., 2006 |
| AB33Kin3G | a2 PnarbW2 PnarbE1, Pkin3-kin3-egfp, bleR, hygR | Schuster et al., 2011c |
| AB33R25G_ChT | a2 PnarbW2 PnarbE1, Prpl25-rpl25-egfp, bleR, hygR/pNomChTub1 | This paper |
| AB33Kin3G_R3Ch3 | a2 PnarbW2 PnarbE1, Pkin3-kin3-egfp, Prps3-rps3-3xmcherry, bleR, hygR, cbxR | This paper |
| poNLS3RFP | Potef-gal4s-3xmrfp, natR | Schuster et al., 2011b |
| popaG2 | Potef-2×pagfp, cbxR | This paper |
| pKin3ts | Pkin3-kin3ts, hygR | Schuster et al., 2011c |
| pomChRab5a | Potef-mcherry-rab5a, natR | Schuster et al., 2011a |
| pERGFP | Potef-calS-egfp-HDEL, cbxR | Wedlich-Söldner et al., 2002a |
| poGRab5a | Potef-egfp-rab5a, natR | Schuster et al., 2011a |
| poPXG | Potef-PX-egfp, cbxR | This paper |
| poGRab4 | Potef-egfp-rab4, cbxR | This paper |
| poGRab7 | Potef-egfp-rab7, cbxR | This paper |
| poG3Rho3 | Potef-3×egfp-rho3, cbxR | This paper |
| pCoGRab5a | Potef-egfp-rab5a, cbxR | This paper |
| pcrgG | Pcrg-egfp, cbxR | This paper |
| pNomChTub1 | Potef-mcherry-tub1, natR | This paper |
a and b, mating type loci; P, promoter; −, fusion; Δ, deletion; hygR, hygromycin resistance; bleR, phleomycin resistance; natR, nourseothricin resistance; cbxR, carboxin resistance; ts, temperature-sensitive allele; NLS, nuclear localization signal of the GAL-4 DNA-binding domain from pC-ACT1 (Takara Bio Inc.); crg, conditional arabinose-induced promoter; otef, constitutive promoter; /, ectopically integrated; E1, W2, genes of the b mating type locus; egfp, enhanced GFP; pagfp, photoactivatable monomeric GFP; mcherry, monomeric cherry; mrfp, monomeric red fluorescent protein; rpl25, ribosomal large subunit protein; rps3, ribosomal small subunit protein; kin3, kinesin-3; dyn2, C-terminal half of the dynein heavy chain; rrm4, RNA-binding protein; rab4, rab5a, and rab7, small endosomal GTPases; rho3, small GTPase; yup1, endosomal t-SNARE; PX, Phox domain from Yup1 (aa 4–148); calS, signal sequence of calreticulin from rabbit (ntl-51); HDEL, ER retention signal; nup107, nucleoporin; tub1, α tubulin.