Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 3;204(3):423–441. doi: 10.1083/jcb.201308028

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Rouvinski et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Live staining: FL PrP^Sc strings are slowly mobile, resembling Ab-clustered PrP^C patches. (A) ScGT1 were treated with PIPLC, labeled with 8B4/secondary Fab (all at 8°C), and followed by time-lapse microscopy at 24°C. Snapshots at t = 0 (red) and t = 5 min (green) are superimposed in c. (B) ScGT1-PPS (a and b) and ScGT1 (c) cells were labeled with 8B4 followed by secondary Fab at 8°C. (b) PrP^C was patched with a tertiary Ab. Cells were then imaged every 400 ms for 24 s at 24°C. Snapshots at t = 0 s (red) and t = 24 s (green) are superimposed. Three types of PrP objects were followed: small dots in cells not patched with the tertiary Ab (a, uninfected), large Ab-induced clusters (b, uninfected), and strings (c, infected). (d–f) Tracks of individual PrP objects over 6 s. Starting point in red (arrow) and end point in green (arrowhead). Many small 8B4 dots (a) were too mobile to allow tracking for the 6-s period. (C) Mean mobility (a) and fluorescence intensity (b) of the three object types measured over 6 s. Both large Ab-induced clusters (blue columns) and strings (red) were mostly immobile, whereas smaller dots (green) in nonpatched samples were very mobile (∼0.4 µm/s).