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. 2014 Feb 3;204(3):423–441. doi: 10.1083/jcb.201308028

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© 2014 Rouvinski et al.

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Figure 7. — Strings do not align with actin fibers or other fibrillar structures. (A) Fixed, permeabilized ScGT1 cells were stained with 8B4 (b and c [red]) and costained with phalloidin to stain actin fibers (a and c [green]). We found no colocalization or alignment between the strings and actin fibers. We also failed to discern higher scale organizations in fibers that would correspond to string clusters. (B and C) Cells were fixed, denatured with GdnSCN, stained with SAF32, and costained with either anti-fibronectin (B) or anti-pacsin 2 (C) Abs. No colocalization was detected.