Fig. 1.

Arachidonic acid metabolite production in vitro by homogenates of chick embryo liver and heart. Representative reverse-phase HPLC chromatograms showing arachidonic acid metabolite production in vitro by 1 mg of liver homogenate (A) and 3 mg of heart homogenate (B) per reaction mixture, after treatment of chick embryos in ovo with TCDD (1 nmol per egg for 24 hours), or for the same amounts of homogenate from solvent controls. Arachidonic acid metabolism was assayed as described in Materials and Methods. Peaks between 0 and 10 minutes are non-P450-mediated polar products. AA, arachidonic acid. Retention times (min) are shown on the abscissas.