Fig. 3.

Effects of TCDD on mRNA levels of enzymes known to affect EETs, DHETs, or HETEs. Mean quantitative real-time PCR results (± S.E.) for total RNA extracted from livers (A) and hearts (B) from chick embryos treated for 24 hours with TCDD or solvent (controls). PCR conditions and specific primers for CYP1A4, CYP1A5, CYP1B1, CYP2C45, CYP2J2, CYP2H1, CYP4B, CYP4V, EPHX1, EPHX2 are described in Materials and Methods Fold changes in mRNAs for genes in livers and hearts of TCDD-treated chick embryos and controls (solvent-treated) are calculated and compared with the values for each gene in control liver for which each mRNA is set at “1” (n = 5 individual organs per treatment group, with three replicates for each organ). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, and ****P ≤ 0.0001; n.s., not significant.