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. 2014 Feb;42(2):294–300. doi: 10.1124/dmd.113.055368

TABLE 1.

Comparison of solid-phase (C18 column) and acetonitrile extraction for LC-MS/MS detection of arachidonic acid metabolites from liver of a chick embryo treated for 24 hours with TCDD as described in Materials and Methods

Means ± S.E. (n = 3 replicates) are shown in the table.

Method EETs DHETS 20-HETE
5,6- 8,9- 11,12- 14,15- 5,6- 8,9- 11,12- 14,15-
ng/g liver
C18 column n.d. 2 ± 0.8 2.4 ± 1.4 n.d. n.d. 2.7 ± 0.9 4 ± 0.4 7 ± 2 4.4 ± 1
Acetonitrile 195 ± 106 9 ± 3 10 ± 1.7 4.2 ± 0.5 5.6 ± 0.5 39 ± 7 133 ± 11 288 ± 37 35 ± 4

n.d., not detected.