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LC-MS/MS MRM chromatograms of nicotine, its metabolites, and ISs. Extracted LLOQ (0.53 ng/ml) with IS (A), extracted ULOQ (504 ng/ml) with IS (B), extracted LQC (3.03 ng/ml) standard (C), extracted MQC (80.6 ng/ml) (D), and extracted HQC (504 ng/ml) (E) from plasma of nonsmokers.
