TABLE 1.
Intraday and interday precision and accuracy of CC and four levels of QC standards for nicotine, nornicotine, cotinine, norcotinine, and trans-3′-hydroxycotinine in pooled human plasma from HIV-negative nonsmokers
| Analyte | Nominal Concentration | Within-Day: CC (n = 4), QC (n = 6) |
Between-Day: CC (n = 6), QC (n = 6) |
||||
|---|---|---|---|---|---|---|---|
| Calculated Concentration | Precision | Accuracy | Calculated Concentration | Precision | Accuracy | ||
| ng/ml | ng/ml | %CV | % | ng/ml | %CV | % | |
| Nicotine | |||||||
| CC standard-1 | 504.00 | 476.57 | 7.6 | 94.6 | 543.9 | 3.8 | 107.9 |
| CC standard-2 | 201.60 | 175.18 | 8.1 | 86.7 | 179.0 | 6.5 | 88.6 |
| CC standard-3 | 80.64 | 78.57 | 5.5 | 97.5 | 91.6 | 9.1 | 113.7 |
| CC standard-4 | 30.64 | 32.63 | 5.5 | 106.6 | 32.3 | 5.9 | 105.7 |
| CC standard-5 | 10.11 | 11.24 | 12.6 | 114.7 | 9.9 | 15.4 | 100.6 |
| CC standard-6 | 3.03 | 3.18 | 12.4 | 102.6 | 2.9 | 1.7 | 92.6 |
| CC standard-7 | 1.06 | 1.07 | 5.1 | 97.3 | 1.1 | 4.9 | 102.2 |
| CC standard-8 | 0.53 | 0.51 | 3.8 | 96.3 | 0.47 | 10.3 | 88.7 |
| HQC | 504.00 | 483.33 | 5.3 | 95.9 | 483.3 | 5.3 | 95.9 |
| MQC | 80.64 | 70.71 | 13.5 | 87.7 | 71.7 | 13.6 | 88.9 |
| LQC | 3.03 | 2.64 | 23.3 | 87.0 | 2.82 | 16.3 | 93.2 |
| LLOQ QC | 1.06 | 1.22 | 31.5 | 114.8 | 1.16 | 27.8 | 109.1 |
| Nornicotine | |||||||
| CC standard-1 | 504.00 | 544.00 | 3.8 | 107.9 | 527.2 | 7.3 | 104.6 |
| CC standard-2 | 201.60 | 179.10 | 6.5 | 88.8 | 179.2 | 6.4 | 88.9 |
| CC standard-3 | 80.64 | 90.38 | 8.9 | 112.1 | 90.7 | 9.3 | 112.4 |
| CC standard-4 | 30.64 | 32.27 | 5.9 | 105.3 | 32.5 | 5.9 | 105.8 |
| CC standard-5 | 10.11 | 10.19 | 14.4 | 100.7 | 10.3 | 13.1 | 102.3 |
| CC standard-6 | 3.03 | 2.99 | 2.2 | 98.6 | 3.0 | 1.6 | 99.1 |
| CC standard-7 | 1.06 | 1.08 | 6.2 | 101.8 | 1.08 | 6.2 | 101.8 |
| CC standard-8 | 0.53 | 0.55 | 23.4 | 103.4 | 0.57 | 22.5 | 106.6 |
| HQC | 504.00 | 483.33 | 5.3 | 95.9 | 483.33 | 5.3 | 95.9 |
| MQC | 80.64 | 70.71 | 13.5 | 87.7 | 71.44 | 14.8 | 88.6 |
| LQC | 3.03 | 2.82 | 16.3 | 93.2 | 2.82 | 16.3 | 93.2 |
| LLOQ QC | 1.06 | 1.17 | 18.4 | 110.7 | 1.24 | 22.4 | 117.0 |
| Cotinine | |||||||
| CC standard-1 | 504.00 | 518.83 | 7.3 | 102.9 | 504.83 | 8.7 | 100.2 |
| CC standard-2 | 201.60 | 179.60 | 6.4 | 89.1 | 181.43 | 6.7 | 90.0 |
| CC standard-3 | 80.64 | 90.55 | 9.2 | 112.2 | 90.11 | 10.0 | 111.7 |
| CC standard-4 | 30.64 | 31.94 | 7.5 | 104.2 | 31.61 | 6.9 | 103.2 |
| CC standard-5 | 10.11 | 10.69 | 11.2 | 105.6 | 10.85 | 10.6 | 107.3 |
| CC standard-6 | 3.03 | 2.98 | 2.1 | 98.4 | 2.99 | 2.7 | 98.8 |
| CC standard-7 | 1.06 | 1.06 | 6.8 | 100.3 | 1.09 | 8.4 | 102.5 |
| CC standard-8 | 0.53 | 1.49 | 14.8 | 81.7 | 1.49 | 148.0 | 281.8 |
| HQC | 504.00 | 480.83 | 5.1 | 95.4 | 478.00 | 4.6 | 94.8 |
| MQC | 80.64 | 70.61 | 12.4 | 87.6 | 72.44 | 7.4 | 89.8 |
| LQC | 3.03 | 2.84 | 14.6 | 93.9 | 2.84 | 14.5 | 93.7 |
| LLOQ QC | 1.06 | 1.19 | 18.7 | 112.3 | 1.21 | 17.6 | 114.5 |
| Norcotinine | |||||||
| CC standard-1 | 504.00 | 545.67 | 3.8 | 108.3 | 545.67 | 3.8 | 108.3 |
| CC standard-2 | 201.60 | 180.60 | 6.0 | 89.6 | 180.60 | 6.0 | 89.6 |
| CC standard-3 | 80.64 | 92.61 | 8.0 | 114.8 | 93.94 | 8.0 | 116.5 |
| CC standard-4 | 30.64 | 32.44 | 6.6 | 105.9 | 31.44 | 6.7 | 102.6 |
| CC standard-5 | 10.11 | 10.19 | 14.4 | 100.7 | 10.35 | 14.5 | 102.4 |
| CC standard-6 | 3.03 | 2.98 | 2.0 | 98.2 | 2.99 | 1.6 | 98.8 |
| CC standard-7 | 1.06 | 0.99 | 7.3 | 93.1 | 1.02 | 11.2 | 96.2 |
| CC standard-8 | 0.53 | 0.64 | 19.9 | 119.8 | 0.62 | 17.9 | 117.3 |
| HQC | 504.00 | 478.00 | 4.7 | 94.8 | 484.50 | 3.7 | 96.1 |
| MQC | 80.64 | 74.27 | 6.1 | 92.1 | 77.27 | 8.6 | 95.8 |
| LQC | 3.03 | 2.95 | 4.0 | 97.5 | 3.00 | 1.9 | 99.1 |
| LLOQ QC | 1.06 | 1.19 | 18.7 | 112.3 | 1.17 | 15.9 | 110.7 |
| Trans-3′-hydroxy cotinine | |||||||
| CC standard-1 | 504.00 | 511.67 | 7.4 | 101.5 | 529.17 | 7.8 | 105.0 |
| CC standard-2 | 201.60 | 183.10 | 6.0 | 90.8 | 182.43 | 6.1 | 90.5 |
| CC standard-3 | 80.64 | 89.61 | 9.4 | 111.1 | 90.27 | 10.2 | 111.9 |
| CC standard-4 | 30.64 | 30.94 | 7.4 | 101.0 | 31.44 | 6.7 | 102.6 |
| CC standard-5 | 10.11 | 11.02 | 9.8 | 109.0 | 10.69 | 12.7 | 105.7 |
| CC standard-6 | 3.03 | 2.98 | 2.9 | 98.2 | 3.01 | 2.2 | 99.3 |
| CC standard-7 | 1.06 | 1.05 | 7.4 | 99.4 | 1.07 | 10.9 | 100.9 |
| CC standard-8 | 0.53 | 2.39 | 116.8 | 451.6 | 0.61 | 19.3 | 115.1 |
| HQC | 504.00 | 484.17 | 3.7 | 96.1 | 484.17 | 3.7 | 96.1 |
| MQC | 80.64 | 73.61 | 7.4 | 91.3 | 75.44 | 10.7 | 93.6 |
| LQC | 3.03 | 3.02 | 0.4 | 99.8 | 3.00 | 1.9 | 98.9 |
| LLOQ QC | 1.06 | 1.12 | 9.2 | 106.0 | 1.11 | 10.1 | 105.0 |
CC, calibration curve.