TABLE 2.
Recovery and matrix effect of four levels of QC standards of nicotine, nornicotine, cotinine, norcotinine, and trans-3′-hydroxycotinine in pooled human plasma from HIV-negative nonsmokers
| Analyte | Nominal Concentration | Recovery (n = 6) |
Matrix Effect (n = 6) |
||
|---|---|---|---|---|---|
| CV | Recovery | CV | Matrix Effect | ||
| ng/ml | % | ||||
| Nicotine | |||||
| HQC | 504.00 | 5.6 | 97.9 | 5.7 | 97.6 |
| MQC | 80.64 | 8.4 | 102.9 | 8.3 | 102.5 |
| LQC | 3.03 | 24.4 | 95.7 | 27.2 | 91.6 |
| Nornicotine | |||||
| HQC | 504.00 | 8.6 | 98.5 | 9.8 | 100.9 |
| MQC | 80.64 | 8.3 | 102.5 | 8.9 | 101.1 |
| LQC | 3.03 | 22.9 | 86.7 | 23.6 | 86.1 |
| Cotinine | |||||
| HQC | 504.00 | 9.7 | 100.9 | 5.6 | 97.9 |
| MQC | 80.64 | 9.1 | 99.5 | 8.4 | 102.9 |
| LQC | 3.03 | 29.3 | 89.6 | 24.4 | 95.7 |
| Norcotinine | |||||
| HQC | 504.00 | 9.7 | 100.9 | 9.6 | 101.2 |
| MQC | 80.64 | 9.1 | 99.5 | 8.7 | 100.2 |
| LQC | 3.03 | 29.3 | 89.6 | 21.0 | 83.2 |
| Trans-3′-hydroxy cotinine | |||||
| HQC | 504.00 | 8.7 | 104.2 | 9.7 | 103.2 |
| MQC | 80.64 | 11.3 | 102.8 | 9.0 | 106.2 |
| LQC | 3.03 | 23.5 | 86.6 | 15.7 | 90.5 |
CC, calibration curve.