Fig. 5.

GNF351 inhibits migration of primary FLS from patients who tested positive for RA. (A) Primary RA-FLS were treated for 12 hours with GNF351 or vehicle alone; after 8 hours, cells were serum-starved for 4 hours. Cells were then plated at 5000 cells per top well of a CIM-Plate 16 in serum-free media with or without GNF351. The bottom wells were filled with either DMEM or DMEM with serum as a chemoattractant. (B) Primary RA-FLS were plated in six-well plates. Cells were treated for 12 hours with 500 nM GNF351 or vehicle alone in triplicate. After 12 hours, a scratch was made in the middle of the well with a sterile p200 pipette tip. At 1 hour post-scratch, cells were retreated with GNF351 or vehicle alone every 12 hours for a total period of 16 hours; wells were photographed immediately after scratch and 16 hours post-scratch. Data points and bars represent mean ± S.E. of three independent determinations. Data were analyzed using one-way ANOVA followed by Tukey’s multiple-comparisons test. ***P < 0.001. Alphabetical characters indicate statistical comparisons between two groups.