FIGURE 3.

Characterization of interstitial lung mononuclear phagocytes after CD11c enrichment. A, The percentage of CD11c⁺ cells recovered from lung tissue digests after CD11c enrichment by magnetic bead positive selection. B, The percentage of CX3CR1⁺ lung mononuclear phagocytes, indicated by EGFP fluorescence, that express either CD11c (left panel) or MHC II (right panel). The percentages indicate a subset of total EGFP⁺ cells. C, The proportion of CX3CR1⁺ lung mononuclear phagocytes that express CD11b. D, The percentage of interstitial CX3CR1⁺ cells that are autofluorescent shows wide variability across samples, ranging from 20 to >90% (left and right panels are representative data from five independent experiments). E, The expression of costimulatory molecules CD80, CD86, and CD40 by CX3CR1⁺ cells is indicated. Cells were obtained from either cx3cr1^GFP/GFP or cx3cr1^GFP/+ mice under basal conditions as no significant differences in expression levels of surface markers were noted between cx3cr1^GFP/GFP and cx3cr1^GFP/+ mice. n = 2 to 3 mice from at least two independent experiments.