AdIP-10-mediated reduction of PC organism burden in CD4-depleted mice. A, Male BALB/c mice were CD4-depleted by i.p. administration of GK1.5 followed by transduction with 5 × 108 PFU of AdIFN or AdEGFP. Three days later, all mice were challenged with 2 × 105 PC cysts and sacrificed at serial intervals for organism burden quantification using TaqMan PCR (n = 5–6; *, denotes p < 0.05 compared with AdEGFP). B, Levels of alveolar and perivascular inflammation were scored by H&E staining of paraffin-embedded lung sections (n = 5; *, denotes p < 0.05 compared with AdEGFP). C, Representative histology at day 28 after PC infection. Lung sections were stained with H&E for inflammation assessment and with Gomori methenamine silver (GMS) for PC infection score.