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. 2012 Jan 4;2011:184295. doi: 10.5402/2011/184295

Figure 4.

Figure 4

Effect of cotreatment of exogenous VEGF and 250 nM Rb1 on cell viability. (a) Cells were seeded at 20,000 per well on Day 0. Cells were co-treated with 250 nM Rb1 and 0, 0.006, 0.06, 0.6 and 6 pg/mL of VEGF respectively. Cells were incubated for 24 hrs and viable cell numbers were determined using methods described previously. Results indicate mean viable cell counts ± SE. Asterisks indicate statistical significance of P ≤ 0.05  (n = 3). (b) % Inhibition of cell viability due to the cotreatment (semi-log plot). The cell count data was compared when the cells were treated with 250 nM Rb1 versus cotreatment with Rb1 and different concentrations of VEGF for 24 hrs. The results indicate the percentage of inhibition with the cotreatment. The % inhibition is calculated by [viable cells with Rb1 treatment − viable cells with VEGF & Rb1 cotreatment]/[viable cells with Rb1 treatment − viable cells with no treatment (control)] using results shown in (a).