Figure 4. Mx2 activity reduces levels of nuclear HIV-1 DNA, is capsid dependent and is more potent in non-dividing cells.
a, Quantitative PCR analysis of reverse transcript (left) and 2-LTR circle (right) abundance in inhibitor treated or Mx2-expressing HOS cells. b, WT or CA-mutant HIV-1 GFP-reporter virus infection of vector or Mx2-expressing HOS cells. Titers are mean+SD, n=3 technical replicates, representative of four experiments. c, Infectivity of WT and N57S CA mutant HIV-1 GFP-reporter viruses in untreated and IFNα-treated THP-1 cells. Titers are mean+SD, n=3 technical replicates, representative of four experiments, fold inhibition is the ratio of the mean titers on untreated and IFNα treated cells. d, HIV-1 GFP reporter virus infection of dividing and non-dividing (aphidicolin treated) vector or Mx2-expressing HOS cell clones.