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Preparations of MSCs. (a) Over 70% clinical trials used MSCs that received 1–5 passages. (b) Doses of trypsin used for passaging were widely varied. (c) Cryopreserved MSCs were used in 35% of clinical trials reviewed. In 47 clinical trials studied, two trials used both fresh cells and cryopreserved cells, thus the total number of reports shown in the graph is 49 (see Supplementary Table 1). (d) It was common to use saline as injection vehicle.
