Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 15;141(4):784–794. doi: 10.1242/dev.097188

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 1. — Retrograde labelling and purification of mouse E11.5 embryonic phrenic MNs by flow cytometry. (A) The phrenic nerve was injected with TMR-dextran in Hb9::GFP trunk explants. Spinal segments are numbered according to the ventral roots that emerge from them (labels on right). C, cervical; T, thoracic. (B) Mid-cervical spinal cord transverse section: phrenic neurons (arrow) are labelled with TMR-dextran (red). The Hb9::GFP transgene labels all MNs; phrenic neurons co-express Pou3f1. Scale bar: 50 μm. (C) TMR⁺ GFP⁺ phrenic neurons and GFP⁺ non-phrenic MNs were isolated from spinal cords (C3-C5 levels) by flow cytometry. (D) TMR⁺ GFP⁺ radial LMC neurons (arrow) in E11.5 cervical spinal cord (C6-C8 levels), following retrograde tracing through the radial nerve. (E) Purification of TMR⁺ GFP⁺ radial LMC neurons by flow cytometry.