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. 2014 Feb 15;141(4):784–794. doi: 10.1242/dev.097188

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 7. — The PN-specific cadherins Cdh10 and Pcdh10 mediate specific clustering of ESC-MNs. (A-C) GFP-only and RFP⁺ GFP⁺ ESC-MNs are randomly mixed at 0 hours. (D-I) Mixed cultures of GFP-only and RFP⁺ GFP⁺ ESC-MNs aggregate within 24 hours. (J) Analysis of like-like clustering of ESC-MNs in mixed cultures (24 hours) by near-neighbour analysis. Bar colours indicate the label of the central cell: red, RFP⁺ GFP⁺ control MNs; green, GFP-only control, iCdh10 or iPcdh10 MNs. P-values were calculated based on the generalised linear model with random effects. The chart shows pooled data from three independent experiments. Eight to ten cell clusters per condition (2267 MNs total) were scored. Scale bars: 50 μm.