Fig. 6.

Both mitotic regeneration and direct transdifferentiation occur in the neonatal mouse cochlea. (A-C) Confocal images of tdTomato⁺ (magenta) HCs (Myo7a, green) that are labeled by EdU (blue) in the apical turn of Pou4f3^DTR/+; Lgr5^CreER/+; ROSA26^{CAG-tdTomato/+} mice at P7 after EdU injections at P3-P5. (D-G) Cross-section focused on the tdTomato⁺/EdU⁺ HC indicated by the arrow in A. Note that GFP expression from the Lgr5^CreER/+ allele is much weaker than EdU labeling. In the apical turn of the same organs, there were also EdU^-/Myo7a⁺/tdTomato⁺ cells (H-J). (I,J) Higher magnification of the boxed region in H. Scale bars: 20 μm.