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. 2013 May 15;140(10):2203–2211. doi: 10.1242/dev.089789

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 2. — Loss of fascin 1 results in fewer melanoblasts by E13.5 and E15.5. (A) Wild-type and fascin 1^-/- embryo pictures and cropped areas used for quantitation (E11.5). (B) Cell number in dorsal-ventral part of E11.5 embryos (wild type, n=8; -/+, n=6; -/-, n=3). Kruskal-Wallis one-way ANOVA test and Mann-Whitney test, n.s., not significant. (C) Wild-type and fascin 1^-/- pictures of whole embryos (E15.5) with cropped, gridded subareas for quantitation. (D,E) Melanoblast number in dorsal-ventral regions of E13.5 (D) and E15.5 (E) embryos. (F) Wild-type and fascin 1^-/- pictures of the forelimbs with cropped, gridded subareas for quantitation. (G,H) Melanoblast number in the forelimbs of E13.5 (G) and E15.5 (H) embryos. Results are expressed as means±s.e.m. (four to six embryos for each genotype), Kruskal-Wallis one-way ANOVA tests were used to compare melanoblast numbers between wild type, -/+ and -/- in each subarea and Mann-Whitney tests were used to compare between two groups, ^*P<0.05; ^**P<0.01; n.s., not significant.