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. 2013 Jul 5;89(3):552–564. doi: 10.1111/mmi.12297

Figure 2.

Figure 2

Gene expression and subcellular localization of G2.A. Northern blot of total RNA extracted from purified asexual parasites (Asx), gametocytes (Gct) and ookinetes (Ook). As a loading control is an ethidium bromide-stained gel of total RNA showing the large and small subunit ribosomal RNA species.B. Confocal microscope bright-field (BF) and green fluorescent (GFP) images of a retort (top) and mature ookinete (bottom). The boxed area is enlarged (zoom), with arrowhead pointing to pore in the centre of the cap structure.C. Ookinete with a typical apical extension (protuberance), showing gap (arrow) between the cap-like structure and the pellicle.D. Immunogold labelling (with silver enhancement) of G2 in an ookinete, showing association with the pellicle.E. Confocal microscope images of a young oocyst showing that the pellicle disappears before the cap-like structure.F. Confocal microscope images of a mature, sporulating oocyst.G. Confocal microscope images of a midgut sporozoite.This figure is available in colour online at http://wileyonlinelibrary.com.