Figure 5.

Myristoylation and detergent solubility of G2.A. Confocal green fluorescence images of a typical G2-KO and IMC1b-KO ookinete, clearly showing the differential localization of the GFP reporter protein fused to amino-terminal amino acids of G2 (myristoylated) and IMC1b (non-myristoylated) respectively.B. Confocal green fluorescence image of a G2-KO oocyst. Hoechst DNA co-staining (blue) shows that GFP is mostly absent from the sporozoite nucleus.C. Western blot analysis of the distribution of G2 in parasite line G2/GFP, and IMC1b in parasite line IMC1b/GFP, in deoxycholate-soluble (S) and deoxycholate-insoluble (I) fractions. T = total protein. The asterisk marks the position of an approximately 65 kDa protein that reacts non-specifically with the anti-GFP antibodies (Saeed et al., ²⁰¹²).D. Distribution of G2 and CTRP in deoxycholate-soluble (S) and deoxycholate-insoluble (I) fractions in the presence (Ca²⁺) or absence (EGTA) of calcium.E. Quantification of CTRP and G2 in the deoxycholate-insoluble fractions in the presence or absence of calcium from (D), expressed as relative amounts (%).F. Distribution of IMC1b in deoxycholate-soluble and -insoluble fractions remains unchanged in response to calcium.This figure is available in colour online at http://wileyonlinelibrary.com.